The Microscope

1. Light microscope
2. Electron microscope

Light microscope can magnify object up to 2000 times. This is the type of microscope that is commonly used in school laboratory.

Electron microscope can magnify a specimen up to 50000 times.

Parts of a light microscope

1. Place the microscope securely on a laboratory bench or table, ensuring it is away from the edge to prevent accidents.
2. Prepare the specimen by mounting it on a slide and covering it with a cover slip.
3. Align the low-power objective lens with the eyepiece.
4. Position the slide on the microscope stage and secure it using stage clips.
5. While looking through the eyepiece, adjust the stage to ensure light passes through the specimen.
6. Use the coarse adjustment knob to bring the specimen into focus.
7. Then, use the fine adjustment knob to sharpen the image.
8. If more detail is required, rotate the nosepiece to switch to a higher-power objective lens.

Student using light microscope

1. Always turn the adjustment knobs slowly, beginning with the coarse adjustment knob.
2. Carry the microscope with both hands—one hand holding the arm and the other supporting the base.
3. When not in use, cover the microscope with a clean cloth and store it in a dry, dust-free environment.
4. Clean dirty lenses using lens tissue or a soft, lint-free cloth.
5. Never place the microscope near the edge of a table or bench to avoid accidental falls.
6. If storing the microscope for a long period, remove the lenses and place them in a desiccator to prevent moisture damage.
7. Avoid touching the microscope with wet hands.
8. Do not touch the surface of mirrors or lenses with your fingers.
9. Lubricate moving parts occasionally to ensure smooth operation.
10. Always use a cover slip to protect the objective lens from direct contact with the specimen.
11. Remove slides from the stage immediately after use to avoid damage or contamination.